| HEPES CAS: 7365-45-9 | ||||
| HEPES is used as biochemisty buffers. It is referred to as a second generation Good's buffer, which displays improved solubility and stability compared to traditional Good's buffers. | ||||
| CAS NO: | 7365-45-9 | |||
| EINECS NO | 230-907-9 | |||
| Ingredients | 2-[4-(2-Hydroxyethyl)-1-piperazinyl]ethanesulfonic Acid | |||
| Content of active ingredients | ≥99.5% | |||
| Grade | AR/ACS equivalent | |||
| Molecular Formula | C8H18N2O4S | |||
| Molecular Weight : | 238.3 | |||
| Buffering Range | 6.8~8.2 | |||
| Packaging | Pkg of 1Kg/10Kg/25Kg | |||
| Storage | Room Temperature | |||
| Items | Specification | |||
| Apparence | White Powder or Crystalline | |||
| APHA (Color, 0.5M NaOH solution) | ≤20 | |||
| Solubility (Turbidity,0.5M NaOH solution) | Clear | |||
| Assay (Titration by HCI) | ≥99.5% | |||
| Infrared Spectrum Complies | Conforms to Structure | |||
| UV Absorbance 260mm 0.1M aqueous solution | absorbance≤0.03 | |||
| UV Absorbance 280mm 0.1M aqueous solution | absorbance≤0.03 | |||
| UV Absorbance 260mm 1.0M aqueous solution | absorbance≤0.05 | |||
| UV Absorbance 290mm 1.0M aqueous solution | absorbance≤0.05 | |||
| pH@ 25°C | 10.6~11.6 | |||
| Loss on drying(105℃ for 3 Hours) Wt% | ≤3.0% | |||
| Water insoluble | ≤0.03% | |||
| Heavy Metals Pb | ≤5ppm | |||
| Iron Fe | ≤5ppm | |||
| Chloride Cl | ≤5ppm | |||
| Calcium Ca | ≤10ppm | |||
| Usage | ||||
| HEPES is used in various biological application such as electrophoresis, protein separation. It is a zwitterion buffer, in general to work with other buffers to provide a stable pH rage. | ||||
